To investigate constituents of Lentinus edodes grown on various, quantitative analyses of ash, crude protein, crude fiber, crude fat, water, nonsoluble nitrogen of the mushrooms were carried out by ordinary methods. The crude fat of the mushroom grown on chestnut tree(1.82%) was greater than that grown on oak (0.76%) or alder (1.17%) tree. The crude fiber of the mushroom grown on oak tree (12.50%) was greater than those grown on alder and chestnut trees, 9.85% and 8.52%, respectively. The crude protein, ash, nonsoluble nitrogen and water contents of the mushrooms grown on the three different trees were similar.