본 실험에서는 한국산 인삼의 주요성분인 Saponin (Panax ginseng C.A. Meyer)이 장내미생물인 E. coli 성장과 TCA 회로에 관여하는 효소중 가장 활성이 크고 중요한 Succinate dehydrogenase활성에 미치는 영향을 관찰함으로써 인삼 Saponin의 생리적 작용의 일부를 밝히고자 실험을 하여 다음과 같은 결과를 얻었다. 1. Succinate에 대한 E. coli Soluble SDH의 Km은 $5.05{ imes}10^{-3}M$이며 assay mixture에서의 인삼 Saponin 농도가 $1{ imes}10^{-1}%{sim}6{ imes}10^{-1}%$에서 Soluble SDH의 활성이 크게 증가되었으며, $4{ imes}10^{-1}%$에서 가장 큰 활성증가를 나타냈으나 $1{ imes}10^{-2}%$ 이하에서는 활성에 별 영향이 없었다. 2. Succinate에 대한 E. coli membrane-bound SDH의 Km 값은 $2.58{ imes}10^{-3}M$이며 인삼 Saponin의 농도가 $1{ imes}1O^{-5}{sim}2{ imes}10^{-1}%$에서는 활성에 큰 영향을 주지 못했고, $4{ imes}10^{-1}%$ 이하에서는 커다란 활성강소 현상을 나타냈다. 3. $1{ imes}10^{-1}%$의 인삼 Saponin이 포함된 medium에서 배양한 E. Coli의 경우, 대조군에 비해 lag phase가 약 2배 긴 반면 $1{ imes}10^{-3}%$의 경우는 약간의 성장촉진 현상을 나타냈다. 4. $1{ imes}10^{-2}{sim}1{ imes}10^{-3}%$의 인삼 Saponin이 포함된 medium에서 배양한 E. coli의 Soluble SDH 활성은 대조군에 비해 낮은 현상을 보였으나, $1{ imes}10^{-1}%$의 경우 약 26% 높은 현상을 나타냈다. 5. E. coli Soluble SDH의 척적 preincubation 온도는 $20^{circ}C$이며, 가장 활성이 좋은 때의 인삼 Saponin의 농도는 $4{ imes}10^{-1}%$이었다. 6. Soluble SDH 활성감소도는 $1{ imes}10^{-1}%$, $1{ imes}10^{-2}%$의 Saponin을 첨가한 경우나 첨가하지 않은 경우냐 거의 비슷하였다.
In this experiment, it was attempted to observe the possible effect of Korean ginseng saponin on the growth and one of the most important enzyme in TCA cycle. succinate dehydrogenase (E.C. 1.3.99.1.), of E. coli w3110 to make a contribution to revealing partially the physiological action of ginseng saponin. The following results were obtained: 1) When the concentrations of ginseng saponin in the assay mixture were between $1{ imes}10^{-1}%$ and $6{ imes}10^{-1}%$, E. coli soluble SDH was highly activated; the maximum activity of E. coli soluble SDH was shown in the presence of $4{ imes}10^{-1}%$ ginseng saponin and its activity was inhibited below $1{ imes}10^{-2}%$. The km value for succinate of E. coli soluble SDH was $5.05{ imes}10^{-3}M$. 2) At $4{ imes}10^{-1}%$ saponin solution. E. coli membrane bound SDH was inhibited significantly but concentrations above $2{ imes}10^{-1}%$ saponin solution were innocuous for the enzyme. The km value for succinate of E. coli membrane bound SDH was $2.58{ imes}10^{-3}M$. 3) When the E. coli were grown in the media containing $1{ imes}10^{-1}%$ ginseng saponin, there was increase in lag phase of E. coli. However, the growth curve of E. coli was similar to that of control when the concentrations of ginseng saponin was between $1{ imes}10^{-2}%$ and $1{ imes}10^{-4}%$. 4) When the E. coli were grown in the media containing $1{ imes}10^{-1}%$ ginseng saponin, the activity of soluble SDH was increased 26% in compare with that of control whereas the activity of soluble SDH seemed to be decreased in E. coli grown in the media containing $1{ imes}10^{-2}%$ and $1{ imes}10^{-3}%$. 5) After preincubation at various temperature. E. coli soluble SDH was highly activated when the concentration of ginseng saponin was $4{ imes}10^{-1}%$. The optimum temperature of E. coli soluble SDH was at $2^{circ}C$ under condition described. 6) After storage with and without ginseng saponins at $2^{circ}C$ or 9, 21, and 27 hours, the activities of soluble SDH were almost similar.
