This study was conducted to investigate the interaction between chemical compositions and agronomic characteristics of perique tobacco leaf. At the topping stage, the thickness of leaf was positively correlated with total sugar($ extrm{0.975}^{**}$), reducing sugar($ extrm{0.975}^{**}$), alkaloid($ extrm{0.518}^{*}$) and petroleum ether extract($ extrm{0.801}^{**}$) contents. The length of upper leaf was negatively correlated with its chemical contents. Total sugar contents of the harvested leaf showed a sigmoid pattern of distribution along the leaf stalk positions.