This experiment was conducted with three varieties (Hicks, Burley 21, Sohyang) and cropping systems (Improved mulching, Mulching, Non mulching) of NC 2326 to analyze growth kinetics by means of growth function involving its velocity and accelerated velocity. The basic growth data were obtained by harvest method at interval of ten days from transplanting to hundred days and analyzed by , regression equation, determinant of matrix, and differentiation. The plot of total dry weight of leaves, stalk and roots per a plant vs. time forms a sigmoid curve and its function fitted logistic satisfactorily. Tobacco plant grows at an accelerated velocity. And growth velocity, symmetric about an inflection point, is proportional to biomass attained and to the difference between biomass attained and the maximum, and to the decrease according to the biomass. Of varieties and cropping systems, the most maximum velocity was 9.58g per day per plant in mulching cultivation of NC 2326 and maximum accelerated velocity was 264mg per $day^2$ per plant in Burley 21.