Ammonium citrate와 ammonium succinate가 고려인삼(Panax ginseng C. A. Meyer) 배양세포의 생육 및 질소성분의 흡수에 미치는 영향을 조사하기 위하여 Murashige and Skoog 배지에서 ammonium nitrate 대신에 농도를 달리하여 ammonium citrate와 ammonium succinate를 첨가한 MS배지에 인삼 callus를 액체 진탕배양하여 다음과 같은 결론을 얻었다. Ammonium citrate 10mM과 ammonium succinate 10mM을 첨가한 배양기에서 생육량, NO$_3$-N 흡수량 및 전질소량이 control보다 많았고, NH$_4$-N 흡수량은 control과 같았다. 또한 각각 5mM씩 첨가한 경우에는 거의 모두 control과 별 차이가 없었다. 결론적으로 ammonium citrate 10mM과 ammonium succinate 10mM첨가한 배양기에서는 NO$_3$-N 등의 흡수를 촉진하여 배양세포의 대사를 촉진시켜 생육량을 증가시키는 것으로 생각된다. 그리하여 ammonium citrate 또는 ammonium succinate의 농도를 조절 공급하면 인삼 배양세포의 증식을 촉진시킬 수 있다고 사료된다.
In order to investigate the effects of ammonium citrate and ammonium succinate on the growth and absorption of nitrogen compounds supplied in the medium, Korean ginseng (Panax ginseng C. A. Meyer) calli were suspension cultured in MS medium with various concentrations of ammonium citrate and ammonium succinate. When Korean ginseng calli were cultured with 10 mM ammonium citrate, 10 mM ammonium succinate, and 10 mM ammonium nitrate (control) in MS media as the nitrogen sources, the growth, $NO_3$-N absorption and total nitrogen content of the Korean ginseng cells were greatest in the ammonium citrate and ammonium succinate concentrations. When Korean ginseng calli were cultured with 5 mM ammonium citrate and 5 mM ammonium succinate, the growth and nitrogen content were superior to those of the control: however, $NO_3$-N and $NH_4$-N absorptions were similar to those of the control. In conclusion, the 10 mM ammonium citrate and 10 mM ammonium succinate may be better able to facilitate the growth and <TEX>$NO_3$</TEX-N utilization and uptake than 5 mM ammonium citrate and 5 mM ammonium succinate concentrations. The concentrations of ammonium citrate and ammonium succinate may be varied in order to obtain the desired effects to facilitate the growth of the gineng callus.
