The purpose of this study is to compare the shape of the apical regions of root canals after instrumentation by various enlarging instruments. 120 extracted, single-rooted human teeth were seperated into 4 experimental groups. Each group provided 30 teeth for experimental use. Group 1 root canals instrumented by the hand-operated Reamer. Group 2 root canals instrumented by the hand-operated H-file. Group 3 root canals instrumented by the hand-operated K-file. Group 4 root canals instrumented by the automated Giromatic file. The results were as follows: 1. The degree of the roundness of the apical regions of root canal walls: 1) Hand-operated instruments were superior to the automated Giromatic file. 2) The Reamer was the most effective instrument among hand-operated instruments 3) There was little difference between the H-file and the K-file. 2. The degree of the smoothness of the apical regions of root canal walls: 1) Root canal walls instrumented by the hand-operated Reamer, H-file and, K-file were generally smooth, and there was little difference among them. 2) Root canal walls instrumented by the automated Giromatic file showed many irregular canal walls. 3. The existance of organic debris in the apical regions of root canals: All organic debris is not removed from root canals, and there was little difference between hand-operated instruments and the automated Giromatic instrument in removing organic debris.