The purpose of this study was to estimate the ability of the marginal fit of Glass ionomer cement. Using the human extracted teeth and 2% acqueous solution of methylene blue, the author investigated the marginal penetration of dye in restorative materials such as Amalgam, Hi-Pol, Glass ionomer cement, Estic microfill and Restodent. The results were as follows. 1. All filling materials showed some degree of marginal penetration. 2. Glass ionomer cement revealed nearly the same microleakage as Estic microfill and Hi-Pol, but showed inferior effect of the marginal seal as compared with Restodent. 3. It is appeared that Amalgam has more effective ability of the marginal fit than the others within a week.