한국산 식물 자원으로 부터 새로운 렉틴 성분을 개발하고 저 55종의 식물 자원을 대상으로 연구한 결과를 토대로 본 연구에서는 16종의 콩과식물 중 개발 가능성이 큰 Phaseolus vulgaris C와 Cercis chinensis로 부터 이 성분의 분리 정제를 시도하였다. DEAE-Sephadex A-50 column과 hydroxyapatite column으로 분리 정제를 하였고, polyacrylamide disc gel electrophoresis, UV spectrophotometry, 그리고 적혈구와의 agglutinating activity등으로 분리 정제된 렉틴을 비교 검토하였다. 본 연구에서 Phaseolus vulgaris 렉틴은 Cercis chinensis 렉틴에 비해 분리 정제 효과가 대단히 양호하였음을 관찰 하였다.
In order to find new lectins from Korean natural products, 55 kinds of plants belonging to 31 families were screened by using several different type of blood cells. Phaseolus vulgaris C(W. K. B.) and Cercis chinenesis of Leguminosae contained strong hemagglutinating proteins. The crude lectins from W. K. B. were purified by DAEA Sephadex A-50 column chromatography. The 0.1 M peak from this procedure demonstrated acceptable criteria, e.g. yield, optical density and hemagglutinating activity and three bands were observed in polyacrylamide disc gel electrophoresis. A further purification was achieved by hydroxyapatite column chromatography and strong hemagglutinating activity was recovered in 0.1 M peak. It was elecrophoresed and found to migrate as a single band. The condition of purification for W. K. B. lectin was encourageable but did not brought agreeable satisfaction to the Cercis chinensis lectin.
