The vibration characteristics of hyoid gears are experimentally investigated with backlash and tooth mesh frequency varied. Experiments are performed with two types of gear sets; well-machined and over-lapped gears. Through the experimental works, a constant torque 0.8kg.m is applied to th gears and a pulse type of trigger signal synchronizing tooth contact is used to measure the tooth mesh frequency and to average the gear vibration signal in the time domain. The time averaged vibration signals are frequency analyzed by a digital signal analyzer. The experimental results show the vibration characteristics of the two different types of gears in relation to backlash amount and mesh frequency variations are well differentiated by harmonic amplitudes and their ratios, and peak-to-peak amplitude of the gear vibration.