This experiment was carried out to study the influence of temperature on the growth rate and nutrient uptake of tobacco seedlings grown on neutralized charcoal bed during temporary transplanting. The highest growth rate was obtained at tile temperature of 26-22-$l8^{circ}C$, probably due to fast establishment of roots in the soil. Seedling growth was better in neutralized charcoal bed than in chaffy charcoal bed at each temperature. Days required for fresh weight to reach 20g/10p1ants were shortened by 9~12days as the temperature increased from 18-14-$l0^{circ}C$ to 26-22-$l8^{circ}C$. Compared with chaffy charcoal bed, days required for fresh weight to reach 20g/10 plants with neutralized charcoal bed were shortened by about 5 days, 4 days, and 2days at 18-14-$l0^{circ}C$, 22-18-$l4^{circ}C$, and 26-22-$l8^{circ}C$, respectively. Three macronutrient (N, $P_2O_5,;K_2O$) contents in seedlings increased with increase of temperature. At each temperature, N and $K_2O$ contents in seedlings of neutralized charcoal bed was higher than those of chaffy charcoal bed, whereas that of $P_2O_5$ was low conversely. Nitrate nitrogen from nitric acid that was used to neutralize chaffy charcoal was maintained to tile end of seedling period, providing the source of nitrate nitrogen for the better seedlings.