When wet-paddy is threshed by conventional auto-fed thresher, the threshed material under the concave is difficult to separate with pneumatic separation only. The development of thresher with an oscillating sieve in addition to the conventional pneumatic separation has been recommanded to improve the separation of grains from straw and chaff having high moisture content. This study was intended to evaluate the separating performance of an oscillating sieve and to obtain the effective operation conditions of the sieve separation. The sieve with oscillating in an elliptical motion was developed and installed in the experimental unit which can simulate the separating operation of the conventional auto-fed thresher. Results of the study are summarized as follows: 1. The flowing velocity of grains on oscillating sieve was increased as the sieve frequency increased but the feed rate did not significantly affect the flowing velocity of grains on sieve. 2. The effects of sieve frequency and the direction of sieve rotation on the separating performance by oscillating sieve show that increasing the frequency of sieve driving crank above the 460 rpm in the same direction of material flow (con-flow) improved the separating performance. 3. The height of grain guide plate and air velocity through the sieve to optimize the separating performance of oscillating sieve were experimentally determined to give about 10cm above the sieve and 7 m/s, respectively.