The author has studied comparatively the sealing quality of conventional Z.O.E. cement, chloropercha and FR cement by means of penetration of 2% methylene blue solution through the apex of human teeth in 112 cases as the time elapsed. The results were as follows; 1. All the specimen, of which dye immersion time is three days, showed dye penetration of low degree unrelated to the kinds of root canal cement and the degree of dye penetration was increased as the time elapsed. 2. Of all the experimental group, the Z.O.E. cement group showed the lowest degree of dye penetration and the FR filling group showed the highest degree of dye penetration. 3. The degree of dye penetration of FR cement group was higher than that of the Z.O.E. cement group but lower than that of the chloropercha group.