쥐의 태아섬유아세포에 MCMV가 감염한 후 16시에 DNA 합성효소가 최대로 유도되었다. 새로 유도된 DNA 합성효소를 DEAE와 phosphocellulose이온교환수지에서 순수 정제하고, 기존숙주 DNA 합성효소와 생화학적 특성 및 분자량을 비교하였다. 최적 pH, $Mg^{++}$이온 요구도, 교환수지에서 eluting되는 형 태와 분자량 등에서 MCMV가 유도한 DNA합성 효소와 기존숙주의 DNA합성효소에 있어 큰차이가 있었다. 이같은 결과는 MCMV가 유도한 합성효소는 MCMV의 유전자에 의하여 생산되었음을 암시한다.
Infection of primary mouse embryo fibroblast (MEF) cell with Murine Cytomegalovirus (MCMV) lead to the induction of viral specific DNA polymerise. MCMV-infected MEF cells were harvested after 16 hours post infection (PI) and the induced-DNA polymerase was extracted and purified by DEAE-cellulose and phosphocellulose column chromatographies. Then the characterization of this enzyme was carried out. Viral enzyme can be distinguished from host cell enzyme by chromatographic behavior, optimal pH, $Mg^{+2}$ ion requirement and molecular weight. The sedimentation coefficients of these two enzymes were determined with the use of glycerol gradient centrifugation. Viral enzyme was 8S, and host enzymes were 10.3 S and 3.4 S. Based on these results, we would like to propose that this MCMV-induced DNA polymerise is viral specific enzyme, in other words, the gene of DNA polymerise, necessary to synthesize the viral DNA, is coded on the viral DNA.
