Aspergillus nidulans의 total tRNA로 부터 4종류의 특정 tRNA를 분리하였다. 모든 tRNA종류를 phenol로 추출한 후 DEAE-cellulose column chromatography로 정제한 tRNA를 2차원 polyacrylamide로 전기영동하여 40여개의 spot로 분리하고 이들을 gel로부터 유출시켰다. 그리고 특청한 tRNA종류를 규명하기 위하여 Aspergillus nidulans에서 황산암모늄분획, DEAE-cellulose column, Sephadex G-50 column.를 사용하여 이들 각 tRNA를 aminoacylation시키고 특정한 $^3H$으로 표지된 아미노산을 받아들이는 tRNA를 분석하여 $tRNA^{Lya}$, $tRNA^{Gly}$, $tRNA^{Val}$를 분리 동정하였다.
tRNAs from fungus Aspergillus nidulans were purified by DEAE-cellulose column chromatography and polyacrylamide gel electrophoresis. These tRNAs were separated into about 40 tRNA spots on two-dimensional polyacrylamide gel electropheresis. Each isolated tRNA was eluted, aminoacylated with $^3{H}$-labeled amino acid by aminoacyl-tRNA synthetase which was prepared through ammonium sulfate fractionation, DEAE-cellulose and Sephadex G-50 column chromatography. We separated 4 kinds of 8 specific tRNAs which were going to be used as probes to find out specific tRNA clones from the total tRNA gene clones of Aspergillus. We report here that those are $tRNA^{Lya}$, $tRNA^{Gly}$, $tRNA^{Val}$, and $tRNA^{Leu}$.
