Thirty seven human maxillary third mooars were injected with china ink, decalcified, cleared, and used in vitro study to determine the number of root canals the frequency and location of lateral canals, canals per root, and frequency of apical deltas. The results were as follows: 1. Less than half of the examined teeth showed 3 roots and 3 canals. 2. The mesiobuccal root was ound to contain a single primary canal in 65.4% of the teeth examined and two canals in 34.6% of the teeth studed. 3. In mesiobuccal roots with two canals, the separated apical foramen appeared 30.8% of the specimen concerned, but no common apical foramen appeared. 4. Of the 106 canals studied, 46.2% of the canals were found to contain lateral canals and these ramifications were mainly located in the apical third of the root.