This experiment was designed to investigate the effect of dietary fibers on serum and liver lipids of cholesterol-fed rats. Forty-two male rats of Sprague-Dawley strain weighed $145{pm}10;g$ were divided into 6 groups, each group receiving a different diet for 6 weeks, i.e., basal diet, basal diet plus 0.5% cholesterol without fiber, basal diet plus 0.5% cholesterol and 5% pectin, basal diet plus 0.5% cholesterol and 5% agar, basal diet plus 0.5% cholesterol and 5% pectin plus tannic acid mixture and basal diet plus 0.5% cholesterol and 5% tannic acid. The lowest net weight gain and digestibility were found in 5% tannic acid-containing group. The weight of kidney, heart and lung was significant by different, however, those of liver and spleen was not significantly different among the groups tested. GOT and GPT of serum were significantly higher in 0.5% cholesterol-containing group without fiber, whereas those of 5% pectin-containing group were significantly lower. Highest total serum protein content was found in 0.5% cholesterol-containing group without fiber. However, albumin and A/G ratio were not significant. The content of total lipid and cholesterol in serum were not significant by different among the groups studied, whereas crude lipid contents of liver in 5% tannic acid and pectin plus tannic acid-containing groups were significantly lower. Cholesterol content in the liver was significantly lower in 5% tannic acid-containing group. Crude lipid and sterol content of feces were significantly higher in 5% pectin-containing group.