This research was carried out study the effect of several relative humidities on the cracking of kernels of chestnuts. The moisture contents of total kernel increased at the higher humidity over 88% relative humidity. but the moisture contents of inner layer was decreased at low relative humidity below 76 %. The starch contents of kernel decreased, but free stgars increased during the total period of storage. The tannin contents increased during total of storage, and the tannin contents of out layer was higher than inner layer because of tannin transfer from astringent shell. As we considered the sprouting ratio, the rotting ratio and the cracking ratio, the optimal relative humidity of chestnut storage was proved 88% relative humidity.