- ??프신의 고정화(固定化)에 관한 연구(硏究)
- ㆍ 저자명
- 박종래,Park. Jong Lae
- ㆍ 간행물명
- 慶北大農學誌
- ㆍ 권/호정보
- 1985년|3권 2호|pp.166-173 (8 pages)
- ㆍ 발행정보
- 경북대학교 농업생명과학대학
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
??신을 DEAE-cellulose, CM-cellulose, Sepharose-4B와 porous glass beads을 사용(使用)하여 고정화(固定化)시켰던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. CM cellulose와 DEAE-cellulose의 ??신 흡착량(吸着量)은 건조된 bead 1 gr에 대하여 각각 10 mg와 15 mg이었고 glutaralde을 사용(使用)함으로서 20 mg와 27 mg로 흡착량(吸着量)이 증가(增加)하였고 효소(酵素)의 활력(活力)은 용해성효소(溶解性酵素)에 비해 각각 22%, 24%로 나타났다. 2. Sepharose-4B을 cyanogen bromide을 사용(使用)하여 활성화(活性化)시킨후 glutaraldehyde을 사용(使用)하여 공유결합을 유도하였던 바 건조된 bead 1 gr에 ??신 19 mg가 통합(統合)되었고 효소(酵素)의 활력(活力)은 23%로 나타났다. 3. Porous glass beads의 표면(表面)을 succineamido - propyl glass beads의 형태(形態)로 유도체를 만든후 ??신을 고정화(固定化)시켰을 때 bead 1 gr가 ??신 40 mg을 통합(統合)할 수 있었고 효소(酵素)의 활력(活力)은 용해성효소(溶解性酵素)에 비해 45%로 나타났다.
Several enzyme immobilization methods has been compared for immobilization of pepsin. Carboxymethyl cellulose and diethylaminoethyl cellulose were activated with Hcl and with NaOH, and were used for immobilization of pepsin. Sepharose-4B was activated cyanogen bromide, and was used for immobilization of pepsin. Porous glass beads were derivatized with 3-aminopropyitrlethoxysilane and with succinicanhydride, and were used for immobilization of pepsin. The results abtained were summarized as follow, 1. 10 mg/gr. dry bead and 15mg/gr. dry bead of pepsin were absorbed to CM-cellulose and DEAE-cellulose, 20 mg/gr. dry bead and 27 mg/gr. dry bead were coupled to CM-cellulose and DEAE-cellulose with glutaraldehyde respectively. Enzyme yields were 22% and 24% of soluble pepsin. 2. 16 mg/gr. dry bead of pepsin was attached to cyanogen bromide activated sepharose-4B, 19mg/gr. dry bead was cross linked to the activated bead with glutaraldehyde. Immobilized enzyme activity was 23% of soluble pepsin. 3. 40 mg/gr. dry bead of pepsin was conjugated to the derivatized glass beads. Immobilized enzyme activity was 45% of soluble pepsin.