저자들은 각종 동물의 소뇌의 계통분류학적 계열에 따른 전자현미경적 연구의 일환으로서 배추흰나비 (pieris repae Linne) 후뇌를 연구대상으로 하여 neuropil을 형성하는 신경종말의 연접형태의 미세구조를 전자 현미경으로 관찰하였던 바 다음과 같은 결론을 얻었다. 배추흰나비의 후뇌에서는 축삭-세포 체연접은 전연 볼 수 없었으며 축삭-수상돌기연접만이 이루어져 있었다. 축삭-수상돌기 연접은대 및 소구형의 연접소포와 타원형의 연접소포를 가진 3형의 축삭종말로서 연접을 하고 있었다.
For the critical point of view. since the phylogenetical approach will give the ultimate value in the correlative study between structural and functional bases, the present study was carried out to make an electron microscopic analyses of the synaptic organizations of the tritocerebrum in the butterfly (Pieris rapae Linne) from Insecta. Samples for electron microscopic study were fixed by immersion with 1% paraformaldehyde 1% glutaraldehyde mixture (phosphate buffer, pH 7. 4), and final tissue blocks trimmed under dissecting microscope were osmicated (1% OsO4). They were embedded in Araldit or Epon 812, and ultra-thin sections were made by LKB-III ultratome following the inspection of semi-thin sections stained with toluidine blue-bolax solutions. Ultra-thin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100B or Hitachi 500 electron microscope We classified the boutons based on the size and shape of synaptic vesicles, the state of synaptic organelles and synaptic junctions. In the tritocerebrum of the butterfly no axo-somatic synapsis was observed and there was only axo-dendritic synapsis. In this case, the synaptic boutons contained three types of vesicles, large and small round, and flat.
