담배 callus 배양시 kinetin과 IAA가 shoot형성과 peroxidase 활성 및 isoenzyme pattern 변화에 미치는 영향을 조사하였다. 담배 callus를 $5{ imes}10^{-7}$ Kinetin/$10^{-8};M$ IAA 또는 $10^{-5};M$ kinetin/$10^{-5}M$ IAA 배지에서 15일 정도 배양하였을 때 shoot형성이 시작되었다. Shoot 형성 초기의 peroxidase 활성은 위의 두 경우 모두 대조군 ($5{ imes}10^{-7};M$ kinetin/$10^{-5};M$ IAA) 보다 증가하였으 며 $10^{-8};M$ IAA 배지에서 배양한 callus 에서는 $10^{-5};M$ kinetin 배지에서 배양한 callus 보다 shoot 형성시 약 두배가량 높은 peroxidase 활성도를 나타냈다. 또한 shoot형성시 $10^{-8};M$ IAA callus에서 peroxidase의 활성도 증가는 cathodic isoperoxidase의 증가와 일치 하였으며 $10^{-5};M$ kinetin callus에서는 anodic isoperoxidase의 증가와 일치 하였다. 이와 같은 사실로 보아 shoot 형성과정에 있어서 total peroxidase의 양보다는 isoperoxidase의 종류가 중요한 역할을 하는 것으로 사료된다.
The effects of various ratios of kinetin and IAA in media on shoot initiation, peroxidase activity and isoperoxidase pattern changes of tobacco callus were examined. Tissues grown on $5{ imes}10^{-7};M$ kinetin/$10^{-8};M$ IAA or $10^{-5};M$ kinetin/$10^{-5};M$ IAA began to form masses of cells in nodular form for shoot initiation after 15 days. The peroxidase activities of shoot intiating calli were higher than the normal non-shoot forming calli at the initial stage of shoot formation. It was also noted that the activity of peroxidase in $10^{-8};M$ IAA cultures was about twice higher than that of $10^{-5}M$ kinetin cultures. The increased enzyme activity in $10^{-8};M$ IAA cultures was due to the increased amount of cathodic isoperoxidases while anodic isoperoxidases were responsible for the increase of peroxidase activity in $10^{-5}M$ kinetin cultures. The results indicate that kinds or groups of isoperoxidases not over-all concentration of enzymes are important in shoot initiating process.
