SJL/J mice의 췌장베타세포를 배양하여 coxsackie $B_3$와 $B_4$, murine cytomegalovirus, mengo vrius, encephalomyocarditis virus의 M variant, mumps vrius, herpes simplex virus type I과 type II등의 virus를 감염시켜, 배양한 췌장 베타세포의 viral induction, viral antigen, viral replication 그리고 세포내와 세포의 insulin치와의 관계등을 연구 검토한 결과, encephalomyocarditis virus의 M variant로 감염시킨 경우에 high viral titer ($8.1{ imes}10^5$ PFU)를 나타냈고, immunofluorescence에 의해서도 coxsackie virus로 감염시킨 경우보다 encephalomyocarditis virus의 M variant로 감염시킨 배양한 췌장베타세포에서 high viral antigen이 발견되었으며 immunoreactive insulin치도 coxsackie virus로 감염시킨 경우보다 encephalomyocarditis virus의 M variant로 감염시킨 경우가 세포내와 세포외 모두에서 현저하게 감소되어 이었다. 이를 종합하여 볼 때 배양한 췌장 베타세포의 insulin분비에 미치는 virus의 영향은 췌장베타세포서의 viral replication능력을 갖은 특정한 virus에 따라 다름을 알 수 있었다.
In vitro infections of murine pancreatic beta cell cultures with coxsackie $B_3$, coxsackie $B_4$, murine cytomegalovirus, mumps virus, EMC-M virus, Herpes simplex virus type I and type II were determined. In vitro infection of the beta cell cultures with EMC-M virus resulted in higher viral titer than the beta cell cultrues infected with other viruses. Double-labelled antibody techniques showed that coxsackie viral antigens were rarely detected in the cultured beta cells which were positive in fluorescence-labelled anti-insulin antibody staining, while high per cent of EMC-M viral antigen was found in those cells. And also its intracellular and extracellular levels of immunoreactive insulin from the beta cell cultures infected with EMC-M virus were more decreased than the beta cell cultures infected with other viruses. It is concluded that the viral infection to murine pancreatic beta cell cultures is restricted to the specific virus which has the ability of replication in the beta cells.
