Cytochrome P-450 (P-450) 유도물질인 phenobarbital (Pb)과 3-methylcholanthrene (3-MC)을 흰쥐 (S.D., male, 150-160 g)에 처리한 후 간 마이 크로좀의 P-450 유도에 따른 Aryl Hydrocarbon Hydroxylase (AHHase) 활성도와 혈 액중의 polyamines 분포 및 농도와의 관계를 검토하였다. P-450-b 유도물질인 Pb 처리는 P-450 함량을 상당히 증가시키면서 대조구의 polyamines 분포 (putrescine, spermidine, spermine)와는 달리 spermidine, spermine의 분포를 나타내지만 총 polyamines 농도는 차이가 없었다. P-450-c 유도물질이며 강력한 발암물질인 3-MC 처리는 putrescine, spermidine의 분포로서 특히 putrescine은 대조구의 10배로, 그리고 총 polyamines 농도는 2배 증가되었으며 AHHase 활성도는 12배 이상 증가폭을 보였다. 이와 같은 현상은 putrescine 생합성에 관여하는 ornithine decarboxylase가 3-MC의 대사산물에 의해 그 활성이 촉진되어 비정상적으로 putrescine이 축적되며 polyamine oxidase가 spermine의 분해를 증가시킨 것으로 P-450-c의 유도에 따른 AHHase 활성도 증가와 polyamines의 생합성 또는 분해와는 밀접한 관계가 있는 것으로 사료된다.
The relationship between polyamines in blood and aryl hydrocarbon hydroxylase (AHHase, EC, 1.14.14.1) activities in hepatic microsomes was observed when rats (Sprague - Dawley, male, 150-160 g) were treated intraperitoneally with phenobarbital (Pb) or 3-methylcholanthrene (3-MC) as potent inducers of cytochrome P-450 (P-450) isoenzymes. In the case of Pb-treatment for an induction of P-450-b, P-450 contents of hepatic microsomes were significantly enhanced and total polyamine levels in blood had no change After administration of 3-MC as a carcinogen and an inducer of P-450-c, the major polyamines measured in blood were putrescine and spermidine and total polyamine level was increased over 2-fold. In particular, putrescine concentration was enhanced drastically when compared with control value (10-fold). These results indicate that only the putrescine was accumulated abnormally because ornithine decarboxylase and polyamine oxidase as enzymes of polyamine synthesis or degradation were stimulated by metabolites of 3-MC treated. On the other hand, hepatic microsomal AHHase activities by the induction of P-450-c were increased over 13-fold. In this respect, a relationship is suggested between AHHase activities enhanced from induction of P-450-c and synthesis or degradation of polyamines.
