연속적으로 대두를 재배한 토양과 신개간지 토양에서 재배한 대두 품종 단엽의 근류로부터 토착대두 근류근 87종을 분리하고 이들을 생장속도에 따라 Bradyrhizobium japonicum (slow-grower) 및 Rhizobium fredii (fast-grower)로 분류한 결과 전자가 55종, 후자가 32종이었다. 이두그룹의 대두 근류균을 질산염 환원특성에 따라 denitrifying fast-grower(F-1), nitrate respiring fast-grower(F-II), denitrifying slow-grower(S-I), 및 nitrate respiring slow-grower (S-II)로 세분 되었으며, 분리균주 총 87종중 S-I, S-II, F-I 및 F-II의 수는 각각 10, 22, 48 및 7종이었다. 이들 각 group 대두 근류균의 균체단백질의 전기영동 pattern상의의 차이를 1차 및 2차원 전기영동으로 분석한 결과 slow 및 fast-grower간에는 현저한 차이를 관찰할 수 있었으나 동일 생장속도를 가진 것 중 denitrifer와 nitrate repirer간에는 큰 차이가 없었다.
Eightyseven strains of indigenous Rhizobia were isolated from the nodule of soybean cultivar, Danyup, cultivated in four different soils sampled from continuously soybean cultivated and newly reclaimed fields. The strains were grouped into Bradyrhizobium japanicum (slow-grower:55 strains) and Rhizobium fredii (fast-grower: 32 strains). The both groups could be divided into two sub-groups according to the denitrification characteristics, that is, denitrifying fast-grower (F-I), nitrate respiring fast-grower (F-II), denitrifying slow-grower (S-I). and nitrate respiring slow-grower (S-II). Among the 87 isolates, F-I, F-II, S-I and S-II sub-groups were 10, 22, 48 and 7 strains, respectively. The one-and two-dimensional polyacrylamide gel electrophoretic pattern of the four sub-groups were compared and discernible difference was observed between fast and slow-grower, but the difference was not discernible between subgroups within the same growth rate group.
