Cytochrome P-450 (P-450) 동위 효소 유도 물질로서 phenobarbital (PB) 또는 3-methylcholanthrene (MC)을 처리한 흰 쥐 strain (Sprague-Dawley, Fischer, Wistar)의 간 마이크로좀에서 총 P-450 함량, aryl hydrocarbon hydroxylase 활성도의 유도 정도 및 P-450 동위 효소의 구성비 등은 strain에 따라 다소 차이를 보였으나, P-450-b type은 PB에 의해, c type은 MC에 의해 주로 유도되었으며, 이들 동위 효소의 기질 특이성, 단일 항체와의 결합에 의한 효소 활성도의 억제, 동위 효소의 항체 결합 부위 등은 strain의 종류와 상관관계가 없는 것으로 보였으며 P-450 동위 효소의 이러한 몇가지 특성들이 strain 사이에서 유전적으로 보존되고 있는 것으로 추정되었다.
After i.p. administration of phenobarbital (PB) or 3-methylcholanthrene (MC) on the adult male rat strains, Wistar, Fischer, and Sprague-Dawley, we compared the properties of hepatic microsomal cytochrome P-450 (P-450) isozymes. Total P-450 contents, degree of induction of aryl hydrocarbon hydroxylase (AHH) activity and isozyme pattern of P-450 were slighly dependent on rat strains. The major form of P-450 isozymes in rats tested was P-450-b in the case of PB-microsomes and that of MC-microsomes was P-450-c. Substrate specificity of the isozymes and the inhibition of activity by the binding of P-450-specific monoclonal antibodies to the isozymes were not associated with rat strains. The results obtained from immunological assay using monoclonal antibodies indicated that the sensitivity of hepatic P-450-dependent AHH activity was rather influenced by the treatment of PB or MC as inducers than by the differences of strain. Thus, it seemed that such characteristics of P-450 isozymes have been genetically conserved among rat strains.
