Rhodotorula marina IFO 0879의 균체생산과 균체 내 유지생산을 위한 최적배양조건에 관하여 조사하였다. 균체량과 유지생산량은 $30^{circ}C$, pH $6.0{sim}7.5$에서 최고량을 나타내었다. $30^{circ}C$에서 8일간 진탕배양하는 동안 질소성분이 어느정도 소모되어 균체가 증식한 후 지방질의 생성이 시작되었으며, 이때 최대균체량은 9.82g/l이고 세포내 지방질은 효모균체에 대하여 최고 35.4%까지 축적하였다. 탄소원으로서 유당(乳糖)과 포도당을, 질소원으로서 ammonium sulfate를 사용하였을 때 균체량과 지방질 함량이 가장 많았다. 배양액의 질소농도가 높을수록 균체 지방함량은 감소하고 균체량이 증가하였으며, 농도가 낮을수록 반대로 되었다. 배양액에 주기적으로 당을 첨가했을 때 초기에는 균체량과 지방질생성량이 대조구보다 적었으나 후기에는 더 많았다. 생산된 지방질의 주지방산은 palmitic acid(20.3%), oleic acid(46.6%) 및 linoleic acid(16.2%)이었다.
The optimum conditions for growth and lipid production of Rhodotorula marina IFO 0879 were investigated. The optimum temperature and pH of cultivation was $30^{circ}C$ and pH 6.0-7.0, respectively. During shaking of the culture for 8 days at $30^{circ}C$, the maximum cell biomass of Rh. marina was 9.82g per liter of the medium, and the lipid content obtained was 35.4(w/w) of the dry cell biomass. Lactose and glucose were the most effective carbon sources for the lipid production. Ammonium sulfate was found to be the most effective nitrogen in culture medium the growth of the yeast was retarded, whereas its growth was favored at high concentrations with decreased lipid yield. When lacose was added during fermentation, in the initial stage cell biomass and lipid production were lower than those of the control, but in the later stage the trend were reversed. The major fatty acids of yeast lipid were palmitic acid(20.3%), oleic acid(46.6%) and linoleic acid(16.2%)
