- 육우수정란 간역동결 및 융해방법에 관한 연구 제칠보. 내동제에 Sucrose 첨가에 따른 액체질소에 미치는 영향
- ㆍ 저자명
- 김중규,강만종,김영훈,장덕지,강민수,김승호
- ㆍ 간행물명
- 가축번식학회지
- ㆍ 권/호정보
- 1988년|12권 2호|pp.84-90 (7 pages)
- ㆍ 발행정보
- 한국동물번식학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
This study was done with mouse embryos to determine effects of the freezing media with or without 10% sucrose, and seeding methods (pincette, no seeding, liquid nitrogen gas phase and copper wire coiled straw) on embryo survival were determined using the FDA test. The summarized results are the following. 1. The FDA score found with copper wire coiled straw, no seeding, pincette and liquid nitrogen gas phase was 3.6, 3.6, 3.3 and 3.0, respectively. There were no significant differences. 2. The embryo score shows higher (P<0.05) survival rate using a freezing medium with sucrose than the one without it. Among the seeding procedure, better resutls are copper wire coiled straw and no seeded. 3. The results suggest that copper wire coiled seeding no seeding be as good as seeding when the mouse embryos were frozen in a liquid nitrogen container using both the freezing and dilution media containing 10% sucrose.