Since the oil shocks of 1970s, the quality of the fuel oil for marine diesel engines has become more degarded than ever. When the poorer quality fuel is burned, carbon residues of the fuel oil cause blockage of the fuel injection valve nozzle and troubles of fuel injection system. The mis-firing of engine occurs due to the decrease of fuel quantity injected, the decrease of compression pressure in the slow speed range, the increase of fuel leaked and the high ignition temperature of degraded fuel etc. This paper is to investigate theoretically the effects of mis-firing on the crankshaft axial vibration of diesel engine. The cylinder pressure in operation is calculated by the computer aided simulation of closed cycle for a large two-stroke diesel engine and also the exciting force of axial vibration and the resonance amplitudes are calculated. And then, the condition of normal state, misfiring and one-cylinder cut-off operation are analyzed. The results of calculations show good agreements with those of the actual measurements.