혈액에서의 theophylline의 HPLC방법과 EMIT방법으로 측정하였다. HPLC방법이 비용이 경제적이고, theophylline과 그 대사물질을 동시에 측정할 수 있었고, 감도도 EMIT법이 $2.5{mu}g/ml$인데 반해 100 ng/ml 까지 검출 가능하였다. HPLC와 EMIT에 의한 theophylline 측정값은 평균 $7.1{pm}3.6{mu}g/ml$과 $8.6{pm}3.4{mu}g/ml$로서 대체적으로 낮은 값을 나타냈다.
The purpose of this article was to assay theophylline in serum by HPLC and EMIT. HPLC offered some advantage over EMIT: firstly, it was more economic, and secondly, it simultaneously could measure theophylline and its metabolites. The limit of detection of theophylline by EMIT was 2.5 ${mu}g/ml$, while HPLC could detect 100 ng/ml of theophylline. In comparison of the EMIT and HPLC assay of theophylline, EMIT values (2.6-16.1 ${mu}g/ml$) were higher than HPLC values (1.5-14.9 ${mu}g/ml$), and the two technique values in 30 samples generally were lower than effective range (10-20 ${mu}g/ml$).
