Rapid and complete epidermal-dermal separation procedure were determined in neonatal rat skin by light microscopic observation and by compairing enzyme activities in the separated epidermis. Microscopic appearance demonstrated the at four different separation procedures used in the study resulted in good separation of epidermis from dermis` heating method (i.e., immersion in 55C water for 30 sec, followed by immersion in 0-4C water) and microwave irradiation for 10 sec were saving time.