In order to study the effects of administration of neomycin on the kidney of Korean black goats, 4.5(group A), 9(group B) or 14(group C) mg/kg, body weight of neomycin was injected intramuscularly turice a day for 84 days. On day 31, unilateral nephreotomy was performed in one animal of each group. The results were as follows: Serum neomycin concentrations were 8.7, 13.8, and 20.2 ${mu}$g/ml 12 hours after injection. Serum BUN and creatinine concentration in nephreotomized goats, regardless of dosage, rapidly increased and came to peak on 3 days after, and then began to decrease to normal range, gradually, In non-nephrectomized goats, regardless of dosage, there was no notable change. In nephreotomized goats, PSP Tl/2 was delayed to the utmost 1 day after nephrectomy, regard-less of dosage, and then began to decrease to normal range, gradually in non-nephrectomized goats, there was not notable change in PSP T$frac{1}{2}$. The intact kidney was markedly enlarged compared with the kidney of non-nephrectomized goats, but there was no microscopic change. It is concluded that administration of neomycin up to 14mg/kg body weight twice a day for 84 days was not nephrotoxic to the Korean black goats.