In order to compare the accessory canal filling effects of the three root canal filling methods with gutta-percha, the author fabricated artificial root canal mold with the first and second accessory canals of chrome-cobalt alloy. After the artificial root canal was filled with gutta-percha by lateral condensation, vertical condensation and low-temperature thermoplasticized gutta-percha injection-molded method, twenty five times respectively, the gutta-percha forced into the first and second accessory canals were measured with caliper for length. The results were as follows: 1. The filling in both accessory canals was most effective in low-temperature thermoplasticized gutta-percha injection-melded method followed in such order as: vertical condensation method and lateral condensation method (p < 0.01). 2. The filling effect of the second accessory canal was more or less higher than that of the first one (p < 0.05). 3. Low-temperature thermoplasticized gutta-percha injection-molded method was fastest in time needed for root canal filling followed by lateral condensation method and vertical condensation method.