$PreS_2-{eta}$-galactosidase 융합단백질을 면역원으로 하여 재조합 $preS_2$ peptide에 대한 단일 클론항체 3종류를 생산하였으며 모두 IgM class로 확인되었다. 그 중 040-01 단일 클론항체를 6% PEG로 처리하여 대장균에서 발현되는 재조합 $preS_2$ peptide를 정제시키기 위한 면역 친화성 column 제조에 이용하였다. Elution buffer로는 0.1M-glycine-HCl(pH 2.5)이 다른 종류의 buffer에 비해 IgM 항체 활성도를 가장 안정되게 유지시켰다. $preS_2$ peptide에 대한 면역 친화성 column의 정제력은 $preS_2$-ABEI.H conjugate를 이용하여 결정하였다.
Three monoclonal antibodies specific to the recombinant $preS_2$ peptide were prepared using $preS_2-{eta}$-galactosidase as an immunogen. All three were classified as immunoglobulin M. One of the three monoclonal antibodies (040-01) was precipitated by 6% PEG and used for the preparation of immunoadsorbent column. This immunoaffinity column was utilized to purify recombinant $preS_2$ peptide which was solubilized and extracted from sonicated E. coli cells. For the storage of this specific antibody, 0.1 M glycine-HCl buffer (pH 2.5) gave the best result. Other buffers tested in this study caused a significant decrease in antibody reactivity. The binding capacity of an immunoadsorbent column for $preS_2$ peptide was determined using $preS_2$-ABEI H conjugate.
