Agrobacterium tumefaciens/binary vector 체제를 이용하여 Escherichia coli 외 베타-glucuronidase 유전자를 담배에 도입한 후 담배에서 베타-glucuronidase 유전자의 발현을 RNA Northern 분석과 베타-glucuronidase 효소활성도를 측정함으로써 확인하였다. 형질전환체인 담배의 callus와 분화된 담배의 잎으로부터 모두 약 2.4 kb의 위치에서 mRNA band가 확인되었으며 아울러 베타-glucuronidase 효소의 활성에 따른 p-nitrophenol 형성에 의한 발색 반응이 관찰되었다.
Bacterial beta-glucuronidase gene (GUS) has been introduced into tobacco by means of Agrobacterium tumefaciens/binary vector system. Northern analysis and enzyme assay for GUS from the regenerated tobacco following A. tumefaciens infection indicated that GUS has been successfully transferred into tobacco and expresed. Northern bands for GUS from the transformed plants were at the mRNA size of ca. 2.4 kb, and spectrophotometric analyses for the enzymatic activities of the plant extracts for beta-glucuronidase showed a peak at ca. 400 nm indicating beta-glucuronidase activities in the transformed plants.
