Fatty acids, sterols and amino acids were confirmed from seeds of Acanthopanax senticosus for. inermis Harms. Fatty acids were isolated from petroleum ether extract and identified by gas liquid chromatography. Its composition was myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid and behenic acid. The composition of sterols that isolated from n-hexane extract were determined by GC/MS. It was confirmed to be a mixture of ${eta}-sitosterol$ and stigmasterol. And several kinds of amino acids-cysteic acid, threonine, serine, proline, glutamic acid, glycine, alanine, valine, isoleucine, leucine, histidine, lysine and arginine-were confirmed from water fraction.