Three fractions-neutral lipids, glycolipids and phospholipids were obtained from total lipids in cultivated mushrooms(Pleurotus ostreatus and Pleurotus sajor-caju) and wild mushrooms (Ramaria botrytis and Lactarius volemus), and their lipid compositions and fatty acid compositions were determined by means of silicic acid column chromatography, thin layer chromatography and gas liquid chromatography. The total lipid contents in mushrooms were $0.32{sim}0.39%$(wet basis). It was found that the contents of phospholipids$(19.4{sim}47.4%)$ and neutral lipids$(32.1{sim}51.9%)$ were high, while that of glycolipids$(14.8{sim}28.7%)$ were low. The main lipid in neutral lipids was triglyceride$(21.2{sim}38.2%)$ followed by free sterol$(21.0{sim}21.9%)$, sterol ester$(10.3{sim}18.6%)$, but the main lipid in neutral lipid of Pleurotus sajor-caju was free fatty acid(34.1%). The main lipid in glycolipids was steryl glycoside$(15.6{sim}29.4%)$ followed by esterified steryl glycoside$(13.4{sim}23.9%)$, monogalactosyl diglyceride$(15.6{sim}24.6%)$. Among the phospholipids, phosphatidyl choline$(36.7{sim}49.5%)$, phosphatidyl ethanolamine$(20.9{sim}29.7%)$, phosphatidyl inositol$(18.4{sim}26.1%)$ were the major components. The major fatty acid of total lipids was linoleic acid followed by palmitic acid, oleic acid. Fatty acid composition was not significantly different among total lipids, neutral lipids, glycolipids and phospholipids contained in tested mushrooms but the main fatty acid in neutral lipid of Ramaria botrytis was oleic acid(48.7%).