In a turbulent premixed flame stabilized by the streamline step, and dominated by a coherent eddy, a flame micro-structure was investigated with analyzing the signals of temperature, the ion current, and schieren phtographs simultaneously. Generally the contours of large scale coherent eddies of schlieren photographs was considered as the flame front, however, the main reaction can be occurred within the eddy as a structure of fine flamelets scale. The surrounding burned gas of flamelets could not propagate to a unburned mixture, obstructing flamelets from propagating to a unburned mixture. Consequently, it could restrain flashback. The main reaction region was found to be located at higher temperature of the burned gas rather than at maximum rms of fluctuating temperature. The peak probability of higher temperature was 6 times greater than that of lower temperature. As it was difficult to infer a flame structure from PDF distribution of the fluctuating temperature in form of bimodal shape, it should be taken into consideration with other informations related to the sensitive flame front, for instance, ion current.