An anatomical study was conducted in order to elucidate the development of primary and secondary ray in Robinia pseudoacacia L. The height of primary ray near pith decreases progressively toward periphery, while the width and number per unit area gradually increase, and then the height, width and number remain somewhat constant. Secondary ray originates from the segmentation of fusiform initials or division of the side or end of fusiform initials at the middle of the first growth layer. And after the height and width of secondary ray increases to some extent, it remains constantly.