This is an experimental study to investigate the characteristics of Diesel spray and Diesel engine performance using ultrasonic injection nozzle (A, B type) and conventional commercial injection nozzle (C type). The results are obtained as follows: 1. SMD and range of size distribution of Diesel spray using the ultrasonic nozzle are smaller than those using the conventional injection nozzle, and spray angle is spread. 2. Because of the difference of the ultrasonic vibration energy transfer in the same condition, the effects of A-type ultrasonic vibration are larger than those of B-type ultrasonic vibration. 3. Attaching the ultrasonic vibrator to the conventional injection nozzle of the Diesel engine, engine performances i.e. BMEP, BSFC, and cylinder peak pressure are improved.