배추흰나비 오령유충, 1일용, 7일용, 탈피직후 성충 그리고 1일 성충의 뇌에서 paraldehyde-fuchsin에 양성인 신경분비세포의 수, 분포 및 분비물질 특성을 조사하였다. medial neurosecretory cell은 5령 유충과 1일용에서 각각 14개와 16개가 관찰되었고 7일용, 탈피직후 성충 및 1일 성충에서는 4개 내지 5개가 관찰되었다. lateral neurosecretory cell은 1일용에서는 관찰되지 않았으나 7일용, 탈피직후 성충 및 1일 성충에서는 뇌반구마다 1개 내지 6개가 존재하였다. tritocerebral neurosecretory cell은 5일 유충에서만 뇌반구마다 2개씩 관찰되었다. 본 실험에서 관찰된 대다수의 신경분비세포는 paraldehyde-fuchsin에 약하게 염색되었으나 1일 성충 뇌의 일부 medial neurosecretory cell은 강하게 염색되었다.
The histochemical characteristics of paraldehyde-fuchsin-positive neurosecretory cells were investigated in the brains of five-instar larva, one-day-old pupa, seven-day-old pupa, adult-just-after-ecdysis and one-day-old adult from Pieris rapae. The fourteen medial neurosecretory cells were identified in the brain of five-instar larva, while the sixteen medial neurosecretory cells were found in the one-day-old pupa. But in later stages they were reduced in number; the eight medial neurosecretory cells in the seven-day-old pupa and one-day-old adult, and the ten medial neurosecretory cells in the adult-just-after-ecdysis. The one to six lateral neurosecretory cells could be found in each cerebral hemisphere of seven-day-old pupa, adult-just-after-ecdysis and one-day-old adult. The two tritocerebral neurosecretory cells appeared only in the five-instar larva. Most of the neurosecretory cells, which have been investigated in this experiment, showed the weak reactivites to paraldehyde-fuchsin, except for a few medial neurosecretory cells which exhibited the strong reactivities.
