The effect of diacylglycerol on glucose release was studied by using 1,2-dioctanoyl-sn-glycerol ($diC_8$), a cell permeable diacylglycerol, in perfused rat liver. The glucose release was increased by $diC_8(50;{mu}M$), and the effect was depended on calcium ions. The increment of glucose release by $diC_8(50;{mu}M$) was inhibited by indomethacin ($50;{mu}M$); the amount of glucose release was almost the same with that of control group. Arachidonic acid($200;{mu}M$) also increased glucose release and the release was inhibited by indomethacin. There was no synergistic effect on glucose release by the combination of $diC_8(50;{mu}M$) and phenylephrine($10;{mu}M$).