토양으로부터 호알칼리성 미생물을 분리하여 동정 한 결과, Bacillus속의 특성을 나타내어 Bacillus sp. SH-8로 명명하였다. Bacillus sp. SH-8은 초기 pH 9.0이상의 알칼리에서만 생육이 가능하였으며 중성 pH에서는 불가능하였다. Bacillus sp. SH-8로부터 U.V.조사에 의해 중성배지에서도 생육이 가능한 변이주를 분리하여 Bacillus sp. SH-8M으로 명명하였으며, 초기 pH 6.9부터 11.0까지 생육이 가능하였다. 따라서 이들 두 균주는 pH 변화에 따른 생육과 효소생산을 비교, 검토하는데 적합한 균주로 생각된다.
An alkalophilic microorganism, SH-8, was isolated from soil samples. It was a Grampositive, catalase-positive, spore-forming and motile rod which was capable of growth in aerobic condition at the initial pH 9.0 or above up to 11.0 and between 15 and $42^{circ}C$ The characteristics of this strain resembled those of the Bacillus group of bacteria. The mutant, Bacillus sp. SH- 8M, was selected from Bacillus sp. SH-8 by U.V. mutagenesis and was able to grow at pH 6.9 up to 11.0. These two strains will be suitable for the comparative study on growth and enzyme production at various pH conditions.
