This study is to investigate the dispersion characteristics of diesel fuel spray in the initial stage of the beginning of the injection under the condition of room temperature and atmospheric pressure. It is difficult to analyse that the diesel fuel spray in diesel engine has unsteady intermittent spray. So author installed a fuel accumulator and an electromagnetic controller in order to keep the constant fuel injection rate with the time variation. With this modified fuel injection system, spray tip penetration, spray angle and initial spray development process are investigated by instantaneous photographic method. The results obtained in this study are as follows : 1) The initial shape of injection of diesel fuel spray shows the form of non-disintegrated intact core, but the formation of ligaments increasingly grows as the time increases. It can also be shown that fine droplets become disintegrated out from the ligaments. 2) The slope of spray tip penetration was changed to two different tendencies with time. The transition point of the slope is shown at the time of around between 0.09 msec and 0.4 msec from the beginning of injection. This is transition time from non-disintegrated intact core to formation of ligaments.