This experiment was carried out in order to study the effect of nitrogen fertilization on the growth of Colonial bentgrass and thatch accumulation under no removing clipping residues. Nitrogen fertilization was applied as 4 levels, 10, 20, 25 and 20gN/m$^2$, respectively. The results were as follows : 1.Response of plant length and dry weight of thatch to N fertilization were significant differences between N levels. It suggested that N fertilization with no clipping residues greatly affected to the growth and thatch accumulation of colonial bentgrass.2.Plant length, the dry weight of clipping residues and coverage were obtained the highest values at 20g N. It was assumed that 20gN/m$^2$ is the limiting N level to obtain the favorable growth of Colonial bentgrass. 3.The dry weight of thatch and lignin content were increased with high nitrogen fertilization level. 4.The dry weight of thatch indicated positive significant correlation with lignin content. 5. The dry weight of thatch per N(THg/N) at 20g~25g/m$^2$ levels were obtained the lowest values than of other levels of N. It may be due to the stimulating of microbial activity by adequate to N fertilizers which increased mineralization of thatch.