We have studied a characterization method of accurate size of spherical particles by fitting experimental light scattering profile to the rigorous theoretical scattering function. An efficient software has been developed for computation of the theoretical scattering function and regression analysis. A light scattering instrument has been built and the necessary data acquisition and analysis are carried out by use of a personal computer with an emphasis on the reduction of analysis and time aiming that this study will be extended toward a development of a practical particle sizing apparatus. The performance of the instrument and the software has been evaluated with latex spheres and found to be satisfactory.