액상 액체 크로마토그래피(HPLC)에 의해 구절초 중 chlorogenic acid (CA), 3,4-o-dicaffeoyl quinic acid (3,4-DCQA), 4,5-o-dicaffeoyl quinic acid (4,5-DCQA)와 linarin의 동시 정량분석법이 새로히 확립되었다. 시료를 20ml 메탄올로 4시간 추출하고 그 추출물을 Sep-Pak $C_18$ cartridge와 용출액으로 4 ml 메탄올-물(1:1)을 사용해서 분리, 정제하였다. HPLC 정량법으로 Bondapak $C_18$ 컬럼(30 cm ${ imes}$ 3.9 mm i.d., 10 ${mu}m$)과 이동상으로 메탄올 -5mM 인산수용액(30:70)의 기울기 용출방식이 사용되었다. 위 정량분석법을 이용해서 시중 구절초 중 그 함량범위는 CA에서 0.35~0.55%, 3,4-DCQA에서 0.46~0.76%, 4,5-DCQA에서 0.077~0.231% 및 linarin에서 0.16~2.72%이었다.
Simultaneous determination of chloragenic acid (CA), 3,4-o-dicafeoyl quinic acid(3,4-DCQA), 4,5-o-dicaffeoyl quinic acid (4,5-DCQA) and linarin in Chrysanthemum sibiricum Fisher was newly established by a reversed-phase high performance liquid chromatography (HPLC). Sample was extracted with 20 ml methanol for 4 hrs. The extract was cleaned up by using Sep-Pak $C_18$ cartridge and 4 ml methanol-$H_2$O(1 : 1) as eluent. Their determination was performed by means of RP-HPLC with Bondapak $C_18$ column (30 cm ${ imes}$ 3.9 mm i.d., 10 ${mu}m$ and gradient elution mode as methanol-5 mM $H_3PO_4$ solution (30 : 70). The established method was applied to various samples purchased. As a result, their content ranges showed to be 0.35~0.55% for CA, 0.46~0.76% for 3,5-DCQA, 0.077~0.23% for 4,5-DCQA and 0.16~2.72% for linarin, respectively.
