Acoustic backscattering from a buble column in water was studied theoretically and experimentally. For theoretical analysis a general scattering theory was used by assuming the bubble column to be lumped element scatterer which can be characterized by its shape, void fraction and dimensions. When the void fraction is less than 1% and the incident frequency is higher than individual bubble resonance frequencies, the experimental results show that the acoustic backscattering from a bubble column depends mainly on the void fraction rather than the individual bubble sizes. It was also theoretically and experimentally observed that the acoustic backscattering levels were increased and their peaks moved to the lower frequency regin by raising the void fraction of bubble column.