B형 간염 바이러스 표면항원에 대한 항체를 가지고 있는 사람이 peripheral blood lymphocyte를 Epstein-Barr virus로 transformation시켰을 때 strong positive clone이 나오는 확률은 최고 23%였으며, cloning과 항체분비 test를 계속했을 때 가장 오랫동안 항체를 분비하는 clone이 3개월간 양성반응을 보였다. Polyclonal B cell activator 중에서 pokeweed mitogen을 in vitro에서 처리했을 때 B형 간염 바이러스 표면항원에 대한 항체를 생산하였으며 $[^3H]$-thymidine incorporation도 증가하였다.
Human peripheral blood lymphocytes (PBL) were isolated from the heparinized blood of antibody positive donors by centrifugation with Ficoll/Paque solution. The PBL were transformed with Epstein-Barr virus and the clones were screened for anti-HBsAg antibody production. Twenty-three percent of the total clones were HBsAg antibody positive. Among them, one clone showed stable antibody production. One of the polyclonal B cell activators, pokeweed mitogen, was effective for production of anti-HBsAg antibody and cell proliferation.
