탈지 가자박으로 부터 free, soluble 및 insoluble bound phenolic acids를 추출하여 식용대두유 기질에서 항산화효과률 0.02%(w/w)의 BHA, BHT의 항산화 효과와 비교하고자 각 기질과 대조구를 $60{pm}1^{circ}C$에서 35일간 저장하면서 매 7일 간격으로 과산화물가, TBA가를 측갱하였다. BHA, BHT와 free, soluble 및 insoluble hound phenolic acids를 첨가한 시험구와 대조구의 과산화물가는 21일의 경우 60, 30, 14, 11, 10, 100이었다. 같은 조건하에서 추출물의 TBA가는 0.150, 0.108, 0.105, 0.073, 0.078, 0.185이었다. 이것으로 보아 phenolic acids는 식용대두유 기질에서 우수한 항산화력을 나타내었다. 분리확인된 phenolic acids는 p-coumaric acid, ferulic acid, phloroglucinol, pyrogallol, vanillic acid, caffeic acid으로 확인되었다.
In this study, antioxidative effectiveness of BHA, BHT at 0.02%(w/w) was compared with those of separated free phenolic acid, ester form and insoluble bound phenolic acid which were extracted from 50 g of Terminalia chebula Retz by MeOH/aceton solvents. Antioxidative effectiveness was measured by peroxide values and TBA values for 7 days, storaging respective substrates and contrast tube at $45{pm}1^{circ}C$ for 35days. Laboratory tubes was added by BHA, BHT, separated free, soluble and insoluble phenolic acid extracts and peroxide value of contrast tube after 21 day storage were 60, 30, 14, 11, 100. On the other hand, at the same conditions, TBA values of each antioxidants were 0.150, 0.108, 0.105, 0.073, 0.078, 0.185. This results remarkably appeared antioxidative effectiveness in meal soybean oil substrates. Phenolic acid separated and identificated were p-coumaric acid, Ferulic acid, Phloroglucinol, Pyrogallol, Vanillic acid and Caffeic acid.
