장내 세균의 생리적 연구를 목적으로 한국인의 장내 상재균을 분리하여 효소 pattern을 관찰하였다. 분리된 Bifidobacterium sp. Int-57은 다른 장내 균종에 비하여 $alpha$-glucosidase, $eta$-glucosidase, $alpha$-galactosidase, $eta$-galactosidase, $eta$-xylosidase, $alpha$-arabinofuranosidase역가가 높았다. Bifidobacterium sp. Int-57의 각 효소 생산에 미치는 탄소원의 영향을 조사하였다. $alpha$-glucosidase는 maltose, $eta$-glucosidase는 cellobiose, $alpha$-galactosidase는 raffinose, 는 lactose, $eta$-xylosidase와 $alpha$-arabinofuranosidase는 xyloserk 각각 최적의 탄소원이었다. 또한 각 효소들의 최적 조건과 pH 안정성을 조사 하였다. $alpha$-glucosidase는 pH 6.0 $40^{circ}C$에서 $eta$-glucosidasessm pH 7.0 50에서, $eta$-galactosidase는 pH 7.0 50에서, $eta$-xylosidase는 pH 6.0 $40^{circ}C$에서, $alpha$-arabinofurnaosidase는 pH 5.0 $50^{circ}C$에서 각각 최적이었다. $alpha$-glucosidase는 pH 4.0~9.0 $eta$-glucosidase는 pH 4.0~7.0 $eta$-galactosidase는 pH 4.0~9.0, $eta$-xylosidase는 pH 4.0~6.0, $alpha$-arabinofuranosidase는 pH 7.0~9.0에서 각각 안정하였다.
In order to study the physiological properties of the intestinal bacteria, we isolated the intestinal bacteria of Koreans and tested the enzymatic patterns. Isolated Bifidobacterium sp. Int-57 had the higher activity of $alpha$-glucosidase, $eta$-glucosidase, $alpha$-galactosidase, $eta$-galactosidase. $eta$-xylosidase and $alpha$-arabinofuranosidase than other intestinal microorganisms. The effect of the carbon sources on the production of each enzymes of Bijidobacterium sp. Int-57 was investigated. The most suitable carbon source for the production of $eta$-glucosidase was maltose, for a-glucosidase cellobiose, for $alpha$-galactosidase raffinose, for $eta$-galactosidase lactose, and for $eta$-xylosidase and $alpha$-arabinofuranosidase xylose, respectively. In addition, we investigated the optimal conditions and pH stability of each crude enzymes. The optimal condition of a-glucosidase was pH 6.0 and $40^{circ}C$. that of Jj-glucosidase pH 7.0 and 50oe, that of $eta$-galactosidase pH 7.0 and $50^{circ}C$, that of $eta$-xylosidase pH 6.0 and $40^{circ}C$ , and that of $alpha$-arabinofuranosidase pH 5.0 and $50^{circ}C$. respectively. a-Glucosidase was stable at pH 4.0-9.0. Jj-glucosidase at pH 4.0-7.0. $eta$-galactosidase at pH 4.0-9.0, $eta$-xylosidase at pH 4.0-6.0, and /3-arabinofuranosidase at pH 7.0-9.0, respectively.
