pH-ISFET와 urease막으로 이루어져 있는 요소센서를 제조하고, 그 감응특성을 조사하였다. pH-ISFET 요소센서는 pH-ISFET의 게이트 위에 glutaraldehyde로 BSA와 urease를 고정화시켜서 제작하였다. pH-ISFET 요소센서의 감응에 미치는 완충용액의 pH 및 농도와 효소의 양의 영향에 대하여 조사하였다. 합성생리염수(pH 7.4)로 만든 요소용액에 대한 감응 특성은 다음과 같다. 곧 직선감응농도범위, 감응기울기(감도) 및 감응시간은 각각 5.0 ${ imes}$ $10^{-4}$ ${sim}$ 5.0 ${ imes}$ $10^{-2}$M, 31.6 mV/decade 및 4${sim}$10분이다.
A preparation method and response characteristics of a urea sensor which consisted of pH-ISFET and urease-immobilized membrane were investigated. The pH-ISFET urea sensor was fabricated by immobilizing BSA and urease with glutaraldehyde on gate of the pH-ISFET. Effects of pH and concentration of working buffer and enzyme load on the pontentiometric response of the pH-ISFET urea sensor were examined. Response characteristics for the determination of urea in synthetic physiological saline solution (pH 7.4) were as follows. That is, the concentration range of linear response, slope of linear response (sensitivity), and response time were 5.0 ${ imes}$ $10^{-4}$ ${sim}$ 5.0 ${ imes}$ $10^{-2}$M, 31.6 mV/decade and 4${sim}$10 min, respectively.
